HPLC

HPLC SOLVENTS AND REAGENTS
Scharlau’s HPLC solvents are in the market since 1982 and are appreciated for its excellent and consistent quality.
In the meantime, we are working with a number of all-glass distillation plants, equipped with multi-plate columns and ability to operate under inert gas atmosphere. This allows us maximum flexibility and the possibility of using one plant per product, thus avoiding the problem of cross-contamination. We employ a number of pre-treatment methods in order to obtain products of outstanding specifications. With a series of new qualities and enhancements of our specifications, we have positioned ourselves in the forefront of maximum performance.

GRADIENT GRADE HPLC SOLVENTS
One of our premium products is the AC0331 Acetonitrile supra gradient HPLC grade, which is a reference product for gradient elution at very low wavelengths. Also available in gradient grade HPLC quality are the solvents Methanol, Ethanol and 2-Propanol.
To meet the stringent demands of gradient applications, our gradient grade HPLC products are quality controlled by a unique Gradient Elution Test. The actual chromatogram is printed on the label as is the actual UV-spectrum.

GPC GRADE TETRAHYDROFURAN
Specially stabilized Tetrahydrofuran is available for GPC (gel permeation chromatography). This Tetrahydrofuran can be used for column-storage, since it does not form damaging peroxides.

FLUORESCENT GRADE ACETONITRILE
Our AC0335 Acetonitrile, fluorescence HPLC grade is gradient and fluorescence controled. It is specially suitable for PAH analysis by HPLC.

Our new 4 l amber glass bottles combine total chemical compatibility with lower cost per litre.

HPLC SOLVENTS CATALOGUE

For those laboratories specialized in HPLC, we have edited a 24 page catalogue covering our full range of HPLC solvents and reagents.
   

ION PAIR REAGENTS AND BUFFERS FOR HPLC

Ion pair chromatography is done when the analytes of interest include ionic compounds that are difficult to separate by reverse phase chromatography. Ion pair reagents are added to the movil phase and combine with ionic analytes. The neutral compounds formed can be easily chromatographed by reverse phase HPLC.
The basic samples can be separated by addition of an alkyl sulphonate ion pair reagent.
The acidic samples can be separated by addition of quaternary ammonium salts or alkyl amine ion pair reagents.

We offer both cationic and anionic ion pair reagents in special HPLC grade qualities to assure trouble-free separations.

DESCRIPTION     1-Butane sulfonic acid, sodium salt     1-Dodecane sulfonic acid, sodium salt     1-Heptane sulfonic acid, 0,1M solution     1-Heptane sulfonic acid, sodium salt    1-Hexane sulfonic acid, 0,1M solution     1-Hexane sulfonic acid, sodium salt     1-Octane sulfonic acid, 0,1M solution     1-Octane sulfonic acid, sodium salt     1-Pentane sufhonic acid, 0,1M solution     1-Pentane sulfonic acid, sodium salt     Tetrabutylammonium bromide     BR0200 Tetrabutylammonium hydroxide, solution 0,1 M, buffered Triethylamine

CAT. NO. AC0601 AC0901 AC1240 AC1241 AC1245 AC1246 AC1700 AC1701 AC1740 AC1741 BR0200 TE0115 TR0218

Our reagents for HPLC are of particularly low UV absorbance. The graph below details a typical result of a product against the catalogue specifications.

U.V. ABSORPTION OF HEXANE SULFONIC ACID

Our HPLC buffers are specially purified to meet very high UV-transparency and are micro-filtered for HPLC suitability.

DESCRIPTION   Ammonium acetate, HPLC grade    Potassium dihydrogen phosphate, HPLC grade   Sodium acetate trihydrate, HPLC grade     Sodium formiate, HPLC grade Sodium hydrogen carbonate, HPLC grade     di-Sodium hydrogen phosphate dihydrate, HPLC grade

CAT. NO. AM0255 PO0261 SO0030 SO0325 SO0130 SO0345